This Fast Software Can Whip You Up an Antibody

By SUSAN LAHEY
Reporter with Silicon Hills News

Monica Berrondo pulls up a digital schematic of an antibody. It resembles remnants of different-colored ribbon stuck together by someone with no sense of design. Each color and bend represents information about the amino acids that comprise the antibody, explains Berrondo, whose company, Macromoltek, made this model possible. If the antibody shown in this image works, it may one day be deployed in the body of a sick person, where it will hunt down and cling to a specific pathogen. There, this colored squiggle may stimulate the immune system to go after the pathogen, mark it as an enemy for other immune system cells to take out, or attack it outright.

Antibodies, unlike many older pharmaceuticals, don’t have to carpet bomb healthy cells to attack specific pathogens. They’re efficient.

It used to take months to design an antibody like this, just to get it to the point where it could be created and tested. With Macromoltek it takes hours.

Researchers who subscribe to Macromoltek can log in to a secure workstation on the website and design a digital antibody using color-coded amino acids to read and track the sequence. The digital model helps researchers evaluate and edit their work, since one error can mess up the entire sequence. In general, Berrondo said, scientists will design maybe 10 antibodies that they hope will destroy a specific pathogen. They choose the best to go to a lab to be created and tested. But the digital design process significantly reduces both the time it takes to create an antibody, and the labor to test multiple iterations. Other companies have similar digital design capabilities, but Berrondo said Macromoltek is the only one she knows of that focuses on the creation of antibodies. The company has another distinction: it is the only one that lets researchers “humanize” antibodies that were originally created using mice.

User friendly science software

Berrondo is a graduate of Johns Hopkins University where she received her PhD in chemical and biomolecular engineering.

“I’ve always been a computer person,” she said. “I did a lot of internships in high school and I hated the applications. I didn’t find them very meaningful. So starting out in college I wondered ‘How can I find a software project that’s meaningful?’” She found the answer in computational biology and molecular modeling, using computers to help researchers solve medical problems.

She also knew she wanted, one day, to start her own company. During her post-graduate work, while she was serving as a software consultant for laboratories, she got the inspiration for Macromoltek.

Most of the software laboratories were working with were difficult to use, for a lot of reasons. Some were too clunky and complicated, some too slow to do the calculations, some didn’t support object design, and so forth. Berrondo found herself returning to the same labs over and over to solve the same problems. So she launched into extensive market research to understand whether labs even used digital design—most didn’t—and if not why. She also asked the ones who did use it what issues they were having. Ultimately, she knew what she needed to make and spent six-to-nine months writing the software.

To find out if her software worked, Berrondo collaborated with the University of Texas on validation studies, designing antibodies and having them produced in the lab. With a dozen or so verified and another dozen in the works, the real world seems to be bearing out her software’s efficacy.

“Their protein modeling capabilities have provided us with a novel and reliable alternative to costly and occasionally challenging wet-lab experiments,” said molecular biologist Curt Hewitt of Signature Science, an Austin based subsidiary of Southwest Research Institute and Macromoltek customer. “Working with them has allowed us to focus on the bigger-picture scientific problems at hand with the confidence that the modeling and technical assumptions we’re operating under are solid.”

Last spring, Macromoltek became a Capital Factory Accelerator company. Since it was the accelerator’s first life science company, adviser Gordon Dougherty said he focused primarily on company strategy, customer acquisition, pricing and similar issues. Berrondo hired her mother, Susana Kaufman, a chemical engineer and software developer with experience in the financial services industry as her CFO.

“Monica is a quiet lion,” he said. “She’s reserved in her demeanor but unbelievably smart. When she asks a question, it’s a question with a purpose. I really respect that about her. I meet with a lot of entrepreneurs who shoot from the hip. She is different, in a good way.”

Though Berrondo has received grants, including recently receiving a National Science Foundation Phase II funding for $750,000 over the next two years, Dougherty said, she has come a long way without receiving investment money.

“She’s waiting until she proves things out to a certain level across her business plan. That’s hard to do. It takes a lot of patience.”

Now if she could design a program for that….